Construct: shRNA TRCN0000010218

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014365.x-638s1c1
DNA Barcode:
CCCTTCCCAGACGACTTGACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HSPB8 (26353)
Gene Description:
heat shock protein family B (small) member 8
Transcript:
RefSeq NM_014365.2 (CURRENT)
Match location:
Position 638 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000010218)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTTCCCAGACGACTTGACA
Hairpin Sequence:
5'-CCGG-CCCTTCCCAGACGACTTGACA-CTCGAG-TGTCAAGTCGTCTGGGAAGGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTTCCCAGACGACTTGACACTCGAGTGTCAAGTCGTCTGGGAAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTTCCCAGACGACTTGACACTCGAGTGTCAAGTCGTCTGGGAAGGG-3'

Other clones with same target sequence:

(none)