Construct: shRNA TRCN0000010226

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014365.x-1033s1c1
DNA Barcode:
GGTCCCTCCTTACTCAACATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HSPB8 (26353)
Gene Description:
heat shock protein family B (small) member 8
Transcript:
RefSeq NM_014365.2 (CURRENT)
Match location:
Position 1033 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000010226)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGTCCCTCCTTACTCAACATT
Hairpin Sequence:
5'-CCGG-GGTCCCTCCTTACTCAACATT-CTCGAG-AATGTTGAGTAAGGAGGGACC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGTCCCTCCTTACTCAACATTCTCGAGAATGTTGAGTAAGGAGGGACCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGTCCCTCCTTACTCAACATTCTCGAGAATGTTGAGTAAGGAGGGACC-3'

Other clones with same target sequence:

  1. TRCN0000352708