Construct: shRNA TRCN0000010258

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033018.x-1095s1c1
DNA Barcode:
GGACCTCAAACACGCCAACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDK16 (5127)
Gene Description:
cyclin dependent kinase 16
Transcript:
RefSeq NM_033018.1 (NON-CURRENT)
Match location:
Position 1095 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000010258)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGACCTCAAACACGCCAACAT
Hairpin Sequence:
5'-CCGG-GGACCTCAAACACGCCAACAT-CTCGAG-ATGTTGGCGTGTTTGAGGTCC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGACCTCAAACACGCCAACATCTCGAGATGTTGGCGTGTTTGAGGTCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGACCTCAAACACGCCAACATCTCGAGATGTTGGCGTGTTTGAGGTCC-3'

Other clones with same target sequence:

  1. TRCN0000342372