Construct: shRNA TRCN0000010299

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000051.x-9530s1c1
DNA Barcode:
TGATGGTCTTAAGGAACATCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ATM (472)
Gene Description:
ATM serine/threonine kinase
Transcript:
RefSeq NM_000051.2 (NON-CURRENT)
Match location:
Position 9530 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000010299)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGATGGTCTTAAGGAACATCT
Hairpin Sequence:
5'-CCGG-TGATGGTCTTAAGGAACATCT-CTCGAG-AGATGTTCCTTAAGACCATCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGATGGTCTTAAGGAACATCTCTCGAGAGATGTTCCTTAAGACCATCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGATGGTCTTAAGGAACATCTCTCGAGAGATGTTCCTTAAGACCATCA-3'

Other clones with same target sequence:

(none)