Construct: shRNA TRCN0000010319

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005215.x-3944s1c1
DNA Barcode:
CATCTCAGCCTGAGCATTCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DCC (1630)
Gene Description:
DCC netrin 1 receptor
Transcript:
RefSeq NM_005215.1 (NON-CURRENT)
Match location:
Position 3944 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000010319)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATCTCAGCCTGAGCATTCTA
Hairpin Sequence:
5'-CCGG-CATCTCAGCCTGAGCATTCTA-CTCGAG-TAGAATGCTCAGGCTGAGATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATCTCAGCCTGAGCATTCTACTCGAGTAGAATGCTCAGGCTGAGATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATCTCAGCCTGAGCATTCTACTCGAGTAGAATGCTCAGGCTGAGATG-3'

Other clones with same target sequence:

(none)