Construct: shRNA TRCN0000010320

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005215.x-4488s1c1
DNA Barcode:
CAGTTCAGAAGGAATAAGCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DCC (1630)
Gene Description:
DCC netrin 1 receptor
Transcript:
RefSeq NM_005215.1 (NON-CURRENT)
Match location:
Position 4488 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000010320)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGTTCAGAAGGAATAAGCAT
Hairpin Sequence:
5'-CCGG-CAGTTCAGAAGGAATAAGCAT-CTCGAG-ATGCTTATTCCTTCTGAACTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGTTCAGAAGGAATAAGCATCTCGAGATGCTTATTCCTTCTGAACTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGTTCAGAAGGAATAAGCATCTCGAGATGCTTATTCCTTCTGAACTG-3'

Other clones with same target sequence:

(none)