Construct: shRNA TRCN0000010324

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001363.x-294s1c1
DNA Barcode:
CACTATACACCTCTTGCATGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DKC1 (1736)
Gene Description:
dyskerin pseudouridine synthase 1
Transcript:
RefSeq NM_001363.2 (NON-CURRENT)
Match location:
Position 294 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000010324)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACTATACACCTCTTGCATGT
Hairpin Sequence:
5'-CCGG-CACTATACACCTCTTGCATGT-CTCGAG-ACATGCAAGAGGTGTATAGTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACTATACACCTCTTGCATGTCTCGAGACATGCAAGAGGTGTATAGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACTATACACCTCTTGCATGTCTCGAGACATGCAAGAGGTGTATAGTG-3'

Other clones with same target sequence:

(none)