Construct: shRNA TRCN0000010345

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005235.x-4915s1c1
DNA Barcode:
CAGTTCTCTGTGGTTCAGGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ERBB4 (2066)
Gene Description:
erb-b2 receptor tyrosine kinase 4
Transcript:
RefSeq NM_005235.1 (NON-CURRENT)
Match location:
Position 4915 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000010345)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGTTCTCTGTGGTTCAGGAA
Hairpin Sequence:
5'-CCGG-CAGTTCTCTGTGGTTCAGGAA-CTCGAG-TTCCTGAACCACAGAGAACTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGTTCTCTGTGGTTCAGGAACTCGAGTTCCTGAACCACAGAGAACTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGTTCTCTGTGGTTCAGGAACTCGAGTTCCTGAACCACAGAGAACTG-3'

Other clones with same target sequence:

(none)