Construct: shRNA TRCN0000010400

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000389.x-526s1c1
DNA Barcode:
CTGATCTTCTCCAAGAGGAAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDKN1A (1026)
Gene Description:
cyclin dependent kinase inhibitor 1A
Transcript:
RefSeq NM_000389.2 (NON-CURRENT)
Match location:
Position 562 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000010400)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGATCTTCTCCAAGAGGAA
Hairpin Sequence:
5'-ACCG-GCTGATCTTCTCCAAGAGGAA-CTCGAG-TTCCTCTTGGAGAAGATCAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGATCTTCTCCAAGAGGAACTCGAGTTCCTCTTGGAGAAGATCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGATCTTCTCCAAGAGGAACTCGAGTTCCTCTTGGAGAAGATCAGC-3'

Other clones with same target sequence:

  1. TRCN0000040124