Construct: shRNA TRCN0000010405

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002895.x-3325s1c1
DNA Barcode:
AGTTGGAGCTCTGTCCTTCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RBL1 (5933)
Gene Description:
RB transcriptional corepressor like 1
Transcript:
RefSeq NM_002895.1 (NON-CURRENT)
Match location:
Position 3325 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000010405)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTTGGAGCTCTGTCCTTCAA
Hairpin Sequence:
5'-CCGG-AGTTGGAGCTCTGTCCTTCAA-CTCGAG-TTGAAGGACAGAGCTCCAACT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTTGGAGCTCTGTCCTTCAACTCGAGTTGAAGGACAGAGCTCCAACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTTGGAGCTCTGTCCTTCAACTCGAGTTGAAGGACAGAGCTCCAACT-3'

Other clones with same target sequence:

(none)