Construct: shRNA TRCN0000010480

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004946.x-4954s1c1
DNA Barcode:
CATACAGACAGATGTCCATCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DOCK2 (1794)
Gene Description:
dedicator of cytokinesis 2
Transcript:
RefSeq NM_004946.1 (NON-CURRENT)
Match location:
Position 4954 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000010480)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATACAGACAGATGTCCATCA
Hairpin Sequence:
5'-CCGG-CATACAGACAGATGTCCATCA-CTCGAG-TGATGGACATCTGTCTGTATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATACAGACAGATGTCCATCACTCGAGTGATGGACATCTGTCTGTATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATACAGACAGATGTCCATCACTCGAGTGATGGACATCTGTCTGTATG-3'

Other clones with same target sequence:

(none)