Construct: shRNA TRCN0000010553

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002314.x-199s1c1
DNA Barcode:
GAAGAACGTATGGGAGAGGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LIMK1 (3984)
Gene Description:
LIM domain kinase 1
Transcript:
RefSeq NM_002314.2 (NON-CURRENT)
Match location:
Position 199 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000010553)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGAACGTATGGGAGAGGAA
Hairpin Sequence:
5'-CCGG-GAAGAACGTATGGGAGAGGAA-CTCGAG-TTCCTCTCCCATACGTTCTTC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGAACGTATGGGAGAGGAACTCGAGTTCCTCTCCCATACGTTCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGAACGTATGGGAGAGGAACTCGAGTTCCTCTCCCATACGTTCTTC-3'

Other clones with same target sequence:

(none)