Construct: shRNA TRCN0000010562

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014602.x-1113s1c1
DNA Barcode:
CGGAGGAGAACTTGCTATATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PIK3R4 (30849)
Gene Description:
phosphoinositide-3-kinase regulatory subunit 4
Transcript:
RefSeq NM_014602.1 (NON-CURRENT)
Match location:
Position 1113 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000010562)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGAGGAGAACTTGCTATATT
Hairpin Sequence:
5'-CCGG-CGGAGGAGAACTTGCTATATT-CTCGAG-AATATAGCAAGTTCTCCTCCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGAGGAGAACTTGCTATATTCTCGAGAATATAGCAAGTTCTCCTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGAGGAGAACTTGCTATATTCTCGAGAATATAGCAAGTTCTCCTCCG-3'

Other clones with same target sequence:

  1. TRCN0000318621