Construct: shRNA TRCN0000010572

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002662.x-520s1c1
DNA Barcode:
CCACTAGAAGACACACGTTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PLD1 (5337)
Gene Description:
phospholipase D1
Transcript:
RefSeq NM_002662.1 (NON-CURRENT)
Match location:
Position 520 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000010572)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACTAGAAGACACACGTTTA
Hairpin Sequence:
5'-CCGG-CCACTAGAAGACACACGTTTA-CTCGAG-TAAACGTGTGTCTTCTAGTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACTAGAAGACACACGTTTACTCGAGTAAACGTGTGTCTTCTAGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACTAGAAGACACACGTTTACTCGAGTAAACGTGTGTCTTCTAGTGG-3'

Other clones with same target sequence:

(none)