Construct: shRNA TRCN0000010602

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006756.x-1750s1c1
DNA Barcode:
TGTTGTAGTTTAGAAGGCTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TCEA1 (6917)
Gene Description:
transcription elongation factor A1
Transcript:
RefSeq NM_006756.1 (NON-CURRENT)
Match location:
Position 1750 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000010602)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGTTGTAGTTTAGAAGGCTTT
Hairpin Sequence:
5'-CCGG-TGTTGTAGTTTAGAAGGCTTT-CTCGAG-AAAGCCTTCTAAACTACAACA-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGTTGTAGTTTAGAAGGCTTTCTCGAGAAAGCCTTCTAAACTACAACATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGTTGTAGTTTAGAAGGCTTTCTCGAGAAAGCCTTCTAAACTACAACA-3'

Other clones with same target sequence:

  1. TRCN0000280085