Construct: shRNA TRCN0000010672

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177559.x-650s1c1
DNA Barcode:
AGAATTTGAGAGGAGGTCCCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CSNK2A1 (1457)
Gene Description:
casein kinase 2 alpha 1
Transcript:
RefSeq NM_177559.1 (NON-CURRENT)
Match location:
Position 650 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000010672)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAATTTGAGAGGAGGTCCCA
Hairpin Sequence:
5'-CCGG-AGAATTTGAGAGGAGGTCCCA-CTCGAG-TGGGACCTCCTCTCAAATTCT-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAATTTGAGAGGAGGTCCCACTCGAGTGGGACCTCCTCTCAAATTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAATTTGAGAGGAGGTCCCACTCGAGTGGGACCTCCTCTCAAATTCT-3'

Other clones with same target sequence:

  1. TRCN0000000609