Construct: shRNA TRCN0000010709

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002706.x-646s1c1
DNA Barcode:
CATCACTACTAACGAAGACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PPM1B (5495)
Gene Description:
protein phosphatase, Mg2+/Mn2+ dependent 1B
Transcript:
RefSeq NM_002706.3 (NON-CURRENT)
Match location:
Position 646 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000010709)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATCACTACTAACGAAGACTT
Hairpin Sequence:
5'-CCGG-CATCACTACTAACGAAGACTT-CTCGAG-AAGTCTTCGTTAGTAGTGATG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATCACTACTAACGAAGACTTCTCGAGAAGTCTTCGTTAGTAGTGATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATCACTACTAACGAAGACTTCTCGAGAAGTCTTCGTTAGTAGTGATG-3'

Other clones with same target sequence:

  1. TRCN0000318554