Construct: shRNA TRCN0000010774

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000125.x-3185s1c1
DNA Barcode:
GCAGGATTGTTGTGGCTACTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ESR1 (2099)
Gene Description:
estrogen receptor 1
Transcript:
RefSeq NM_000125.1 (NON-CURRENT)
Match location:
Position 3185 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000010774)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGGATTGTTGTGGCTACTA
Hairpin Sequence:
5'-CCGG-GCAGGATTGTTGTGGCTACTA-CTCGAG-TAGTAGCCACAACAATCCTGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGGATTGTTGTGGCTACTACTCGAGTAGTAGCCACAACAATCCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGGATTGTTGTGGCTACTACTCGAGTAGTAGCCACAACAATCCTGC-3'

Other clones with same target sequence:

(none)