Construct: shRNA TRCN0000010829

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002795.x-303s1c1
DNA Barcode:
GTTGAAGGAAGGTCGGCAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PSMB3 (5691)
Gene Description:
proteasome 20S subunit beta 3
Transcript:
RefSeq NM_002795.2 (NON-CURRENT)
Match location:
Position 303 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000010829)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTGAAGGAAGGTCGGCAGAT
Hairpin Sequence:
5'-CCGG-GTTGAAGGAAGGTCGGCAGAT-CTCGAG-ATCTGCCGACCTTCCTTCAAC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTGAAGGAAGGTCGGCAGATCTCGAGATCTGCCGACCTTCCTTCAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTGAAGGAAGGTCGGCAGATCTCGAGATCTGCCGACCTTCCTTCAAC-3'

Other clones with same target sequence:

  1. TRCN0000349552