Construct: shRNA TRCN0000010869

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012170.x-758s1c1
DNA Barcode:
GTGTGGTCCTTGTCTTTGGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FBXO22 (26263)
Gene Description:
F-box protein 22
Transcript:
RefSeq NM_012170.2 (NON-CURRENT)
Match location:
Position 758 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000010869)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGTGGTCCTTGTCTTTGGTT
Hairpin Sequence:
5'-CCGG-GTGTGGTCCTTGTCTTTGGTT-CTCGAG-AACCAAAGACAAGGACCACAC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGTGGTCCTTGTCTTTGGTTCTCGAGAACCAAAGACAAGGACCACACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGTGGTCCTTGTCTTTGGTTCTCGAGAACCAAAGACAAGGACCACAC-3'

Other clones with same target sequence:

(none)