Construct: shRNA TRCN0000010894

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022039.x-1106s1c1
DNA Barcode:
GATGAGGACGTTTGCCACTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FBXW4 (6468)
Gene Description:
F-box and WD repeat domain containing 4
Transcript:
RefSeq NM_022039.2 (NON-CURRENT)
Match location:
Position 1106 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000010894)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGAGGACGTTTGCCACTTT
Hairpin Sequence:
5'-CCGG-GATGAGGACGTTTGCCACTTT-CTCGAG-AAAGTGGCAAACGTCCTCATC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGAGGACGTTTGCCACTTTCTCGAGAAAGTGGCAAACGTCCTCATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGAGGACGTTTGCCACTTTCTCGAGAAAGTGGCAAACGTCCTCATC-3'

Other clones with same target sequence:

(none)