Construct: shRNA TRCN0000010963

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032718.2-429s1c1
DNA Barcode:
GCCACCAATGTGTTTCTGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MFSD9 (84804)
Gene Description:
major facilitator superfamily domain containing 9
Transcript:
RefSeq NM_032718.2 (NON-CURRENT)
Match location:
Position 429 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000010963)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCACCAATGTGTTTCTGTTT
Hairpin Sequence:
5'-CCGG-GCCACCAATGTGTTTCTGTTT-CTCGAG-AAACAGAAACACATTGGTGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCACCAATGTGTTTCTGTTTCTCGAGAAACAGAAACACATTGGTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCACCAATGTGTTTCTGTTTCTCGAGAAACAGAAACACATTGGTGGC-3'

Other clones with same target sequence:

(none)