Construct: shRNA TRCN0000010976

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005504.3-1367s1c1
DNA Barcode:
CCTGTGTTGTTTGCCCAGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BCAT1 (586)
Gene Description:
branched chain amino acid transaminase 1
Transcript:
RefSeq NM_005504.3 (NON-CURRENT)
Match location:
Position 1367 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000010976)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGTGTTGTTTGCCCAGTTT
Hairpin Sequence:
5'-CCGG-CCTGTGTTGTTTGCCCAGTTT-CTCGAG-AAACTGGGCAAACAACACAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGTGTTGTTTGCCCAGTTTCTCGAGAAACTGGGCAAACAACACAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGTGTTGTTTGCCCAGTTTCTCGAGAAACTGGGCAAACAACACAGG-3'

Other clones with same target sequence:

(none)