Construct: shRNA TRCN0000010998

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002746.1-480s1c1
DNA Barcode:
GCAGCTGAGCAATGACCATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAPK3 (5595)
Gene Description:
mitogen-activated protein kinase 3
Transcript:
RefSeq NM_002746.1 (NON-CURRENT)
Match location:
Position 480 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000010998)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGCTGAGCAATGACCATAT
Hairpin Sequence:
5'-CCGG-GCAGCTGAGCAATGACCATAT-CTCGAG-ATATGGTCATTGCTCAGCTGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGCTGAGCAATGACCATATCTCGAGATATGGTCATTGCTCAGCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGCTGAGCAATGACCATATCTCGAGATATGGTCATTGCTCAGCTGC-3'

Other clones with same target sequence:

(none)