Construct: shRNA TRCN0000011034

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_053274.1-747s1c1
DNA Barcode:
CCCTTTGCTGACAGCACAATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GLMN (11146)
Gene Description:
glomulin, FKBP associated protein
Transcript:
RefSeq NM_053274.1 (NON-CURRENT)
Match location:
Position 747 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000011034)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTTTGCTGACAGCACAATT
Hairpin Sequence:
5'-CCGG-CCCTTTGCTGACAGCACAATT-CTCGAG-AATTGTGCTGTCAGCAAAGGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTTTGCTGACAGCACAATTCTCGAGAATTGTGCTGTCAGCAAAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTTTGCTGACAGCACAATTCTCGAGAATTGTGCTGTCAGCAAAGGG-3'

Other clones with same target sequence:

  1. TRCN0000337525
  2. TRCN0000342547