Construct: shRNA TRCN0000011041

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001633.2-1027s1c1
DNA Barcode:
CGGTAACAACTTCGTCACAGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AMBP (259)
Gene Description:
alpha-1-microglobulin/bikunin precursor
Transcript:
RefSeq NM_001633.2 (NON-CURRENT)
Match location:
Position 1027 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000011041)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGTAACAACTTCGTCACAGA
Hairpin Sequence:
5'-CCGG-CGGTAACAACTTCGTCACAGA-CTCGAG-TCTGTGACGAAGTTGTTACCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGTAACAACTTCGTCACAGACTCGAGTCTGTGACGAAGTTGTTACCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGTAACAACTTCGTCACAGACTCGAGTCTGTGACGAAGTTGTTACCG-3'

Other clones with same target sequence:

(none)