Construct: shRNA TRCN0000011061

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_182734.1-347s1c1
DNA Barcode:
CCCATTTGAATCTCGTGGCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PLCB1 (23236)
Gene Description:
phospholipase C beta 1
Transcript:
RefSeq NM_182734.1 (NON-CURRENT)
Match location:
Position 347 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000011061)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCATTTGAATCTCGTGGCTT
Hairpin Sequence:
5'-CCGG-CCCATTTGAATCTCGTGGCTT-CTCGAG-AAGCCACGAGATTCAAATGGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCATTTGAATCTCGTGGCTTCTCGAGAAGCCACGAGATTCAAATGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCATTTGAATCTCGTGGCTTCTCGAGAAGCCACGAGATTCAAATGGG-3'

Other clones with same target sequence:

(none)