Construct: shRNA TRCN0000011095

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007106.2-757s1c1
DNA Barcode:
GAGAGACATTACCAGAGCCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
UBL3 (5412)
Gene Description:
ubiquitin like 3
Transcript:
RefSeq NM_007106.2 (NON-CURRENT)
Match location:
Position 757 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000011095)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGAGACATTACCAGAGCCAA
Hairpin Sequence:
5'-CCGG-GAGAGACATTACCAGAGCCAA-CTCGAG-TTGGCTCTGGTAATGTCTCTC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGAGACATTACCAGAGCCAACTCGAGTTGGCTCTGGTAATGTCTCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGAGACATTACCAGAGCCAACTCGAGTTGGCTCTGGTAATGTCTCTC-3'

Other clones with same target sequence:

(none)