Construct: shRNA TRCN0000011157

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145809.1-3344s1c1
DNA Barcode:
CCCAGACATTAGGAGTTCATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
USP32P2 (220594)
Gene Description:
ubiquitin specific peptidase 32 pseudogene 2
Transcript:
RefSeq NM_145809.1 (NON-CURRENT)
Match location:
Position 3344 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000011157)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAGACATTAGGAGTTCATA
Hairpin Sequence:
5'-CCGG-CCCAGACATTAGGAGTTCATA-CTCGAG-TATGAACTCCTAATGTCTGGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAGACATTAGGAGTTCATACTCGAGTATGAACTCCTAATGTCTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAGACATTAGGAGTTCATACTCGAGTATGAACTCCTAATGTCTGGG-3'

Other clones with same target sequence:

(none)