Construct: shRNA TRCN0000011198

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_170662.2-1206s1c1
DNA Barcode:
CCCTTATTTCAAGCCCTGATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CBLB (868)
Gene Description:
Cbl proto-oncogene B
Transcript:
RefSeq NM_170662.2 (NON-CURRENT)
Match location:
Position 1206 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000011198)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTTATTTCAAGCCCTGATT
Hairpin Sequence:
5'-CCGG-CCCTTATTTCAAGCCCTGATT-CTCGAG-AATCAGGGCTTGAAATAAGGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTTATTTCAAGCCCTGATTCTCGAGAATCAGGGCTTGAAATAAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTTATTTCAAGCCCTGATTCTCGAGAATCAGGGCTTGAAATAAGGG-3'

Other clones with same target sequence:

(none)