Construct: shRNA TRCN0000011272

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012125.2-491s1c1
DNA Barcode:
CCAGCTCAAGACAGTTAACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CHRM5 (1133)
Gene Description:
cholinergic receptor muscarinic 5
Transcript:
RefSeq NM_012125.2 (NON-CURRENT)
Match location:
Position 491 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000011272)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGCTCAAGACAGTTAACAA
Hairpin Sequence:
5'-CCGG-CCAGCTCAAGACAGTTAACAA-CTCGAG-TTGTTAACTGTCTTGAGCTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGCTCAAGACAGTTAACAACTCGAGTTGTTAACTGTCTTGAGCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGCTCAAGACAGTTAACAACTCGAGTTGTTAACTGTCTTGAGCTGG-3'

Other clones with same target sequence:

(none)