Construct: shRNA TRCN0000011275

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004072.1-561s1c1
DNA Barcode:
CCCTGATTATTTAGACTCCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CMKLR1 (1240)
Gene Description:
chemerin chemokine-like receptor 1
Transcript:
RefSeq NM_004072.1 (NON-CURRENT)
Match location:
Position 561 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000011275)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTGATTATTTAGACTCCAT
Hairpin Sequence:
5'-CCGG-CCCTGATTATTTAGACTCCAT-CTCGAG-ATGGAGTCTAAATAATCAGGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTGATTATTTAGACTCCATCTCGAGATGGAGTCTAAATAATCAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTGATTATTTAGACTCCATCTCGAGATGGAGTCTAAATAATCAGGG-3'

Other clones with same target sequence:

(none)