Construct: shRNA TRCN0000011493

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005279.2-1303s1c1
DNA Barcode:
CGACTCCACTTTCATAGTTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GPR1 (2825)
Gene Description:
G protein-coupled receptor 1
Transcript:
RefSeq NM_005279.2 (NON-CURRENT)
Match location:
Position 1303 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000011493)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGACTCCACTTTCATAGTTAT
Hairpin Sequence:
5'-CCGG-CGACTCCACTTTCATAGTTAT-CTCGAG-ATAACTATGAAAGTGGAGTCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGACTCCACTTTCATAGTTATCTCGAGATAACTATGAAAGTGGAGTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGACTCCACTTTCATAGTTATCTCGAGATAACTATGAAAGTGGAGTCG-3'

Other clones with same target sequence:

(none)