Construct: shRNA TRCN0000011497

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005279.2-1045s1c1
DNA Barcode:
CCATCCTTTATGTCCTAATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GPR1 (2825)
Gene Description:
G protein-coupled receptor 1
Transcript:
RefSeq NM_005279.2 (NON-CURRENT)
Match location:
Position 1045 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000011497)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATCCTTTATGTCCTAATTA
Hairpin Sequence:
5'-CCGG-CCATCCTTTATGTCCTAATTA-CTCGAG-TAATTAGGACATAAAGGATGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATCCTTTATGTCCTAATTACTCGAGTAATTAGGACATAAAGGATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATCCTTTATGTCCTAATTACTCGAGTAATTAGGACATAAAGGATGG-3'

Other clones with same target sequence:

(none)