Construct: shRNA TRCN0000011529

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153834.1-1136s1c1
DNA Barcode:
CCGGAACTGTACCTTGGTTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ADGRG4 (139378)
Gene Description:
adhesion G protein-coupled receptor G4
Transcript:
RefSeq NM_153834.1 (NON-CURRENT)
Match location:
Position 1136 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000011529)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGGAACTGTACCTTGGTTTA
Hairpin Sequence:
5'-CCGG-CCGGAACTGTACCTTGGTTTA-CTCGAG-TAAACCAAGGTACAGTTCCGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGGAACTGTACCTTGGTTTACTCGAGTAAACCAAGGTACAGTTCCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGGAACTGTACCTTGGTTTACTCGAGTAAACCAAGGTACAGTTCCGG-3'

Other clones with same target sequence:

(none)