Construct: shRNA TRCN0000011535

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153835.1-2400s1c1
DNA Barcode:
GCGGAACAAGATCTCCTATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ADGRF3 (165082)
Gene Description:
adhesion G protein-coupled receptor F3
Transcript:
RefSeq NM_153835.1 (NON-CURRENT)
Match location:
Position 2400 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000011535)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGGAACAAGATCTCCTATTT
Hairpin Sequence:
5'-CCGG-GCGGAACAAGATCTCCTATTT-CTCGAG-AAATAGGAGATCTTGTTCCGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGGAACAAGATCTCCTATTTCTCGAGAAATAGGAGATCTTGTTCCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGGAACAAGATCTCCTATTTCTCGAGAAATAGGAGATCTTGTTCCGC-3'

Other clones with same target sequence:

(none)