Construct: shRNA TRCN0000011536

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153835.1-3001s1c1
DNA Barcode:
CCTCATTACATCTTCACCATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ADGRF3 (165082)
Gene Description:
adhesion G protein-coupled receptor F3
Transcript:
RefSeq NM_153835.1 (NON-CURRENT)
Match location:
Position 3001 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000011536)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCATTACATCTTCACCATT
Hairpin Sequence:
5'-CCGG-CCTCATTACATCTTCACCATT-CTCGAG-AATGGTGAAGATGTAATGAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCATTACATCTTCACCATTCTCGAGAATGGTGAAGATGTAATGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCATTACATCTTCACCATTCTCGAGAATGGTGAAGATGTAATGAGG-3'

Other clones with same target sequence:

(none)