Construct: shRNA TRCN0000011554

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032777.6-490s1c1
DNA Barcode:
CCGACTAAACATATCTGGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ADGRA2 (25960)
Gene Description:
adhesion G protein-coupled receptor A2
Transcript:
RefSeq NM_032777.6 (NON-CURRENT)
Match location:
Position 490 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000011554)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGACTAAACATATCTGGAAA
Hairpin Sequence:
5'-CCGG-CCGACTAAACATATCTGGAAA-CTCGAG-TTTCCAGATATGTTTAGTCGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGACTAAACATATCTGGAAACTCGAGTTTCCAGATATGTTTAGTCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGACTAAACATATCTGGAAACTCGAGTTTCCAGATATGTTTAGTCGG-3'

Other clones with same target sequence:

(none)