Construct: shRNA TRCN0000011562

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020455.3-3849s1c1
DNA Barcode:
CCTATCTTACATCCAAATCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ADGRG6 (57211)
Gene Description:
adhesion G protein-coupled receptor G6
Transcript:
RefSeq NM_020455.3 (NON-CURRENT)
Match location:
Position 3849 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000011562)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTATCTTACATCCAAATCTA
Hairpin Sequence:
5'-CCGG-CCTATCTTACATCCAAATCTA-CTCGAG-TAGATTTGGATGTAAGATAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTATCTTACATCCAAATCTACTCGAGTAGATTTGGATGTAAGATAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTATCTTACATCCAAATCTACTCGAGTAGATTTGGATGTAAGATAGG-3'

Other clones with same target sequence:

  1. TRCN0000273865