Construct: shRNA TRCN0000011606

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032551.2-228s1c1
DNA Barcode:
GACCAACTTCTACATCGCCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KISS1R (84634)
Gene Description:
KISS1 receptor
Transcript:
RefSeq NM_032551.2 (NON-CURRENT)
Match location:
Position 228 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000011606)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACCAACTTCTACATCGCCAA
Hairpin Sequence:
5'-CCGG-GACCAACTTCTACATCGCCAA-CTCGAG-TTGGCGATGTAGAAGTTGGTC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACCAACTTCTACATCGCCAACTCGAGTTGGCGATGTAGAAGTTGGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACCAACTTCTACATCGCCAACTCGAGTTGGCGATGTAGAAGTTGGTC-3'

Other clones with same target sequence:

(none)