Construct: shRNA TRCN0000011618

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005682.4-1981s1c1
DNA Barcode:
CCTGGTGTTTCTGTTCAACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ADGRG1 (9289)
Gene Description:
adhesion G protein-coupled receptor G1
Transcript:
RefSeq NM_005682.4 (NON-CURRENT)
Match location:
Position 1981 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000011618)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGGTGTTTCTGTTCAACAT
Hairpin Sequence:
5'-CCGG-CCTGGTGTTTCTGTTCAACAT-CTCGAG-ATGTTGAACAGAAACACCAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGGTGTTTCTGTTCAACATCTCGAGATGTTGAACAGAAACACCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGGTGTTTCTGTTCAACATCTCGAGATGTTGAACAGAAACACCAGG-3'

Other clones with same target sequence:

  1. TRCN0000318767