Construct: shRNA TRCN0000011698

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021634.1-2010s1c1
DNA Barcode:
GCCCATTAACAGTGCTTTGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RXFP1 (59350)
Gene Description:
relaxin family peptide receptor 1
Transcript:
RefSeq NM_021634.1 (NON-CURRENT)
Match location:
Position 2010 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000011698)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCATTAACAGTGCTTTGAA
Hairpin Sequence:
5'-CCGG-GCCCATTAACAGTGCTTTGAA-CTCGAG-TTCAAAGCACTGTTAATGGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCATTAACAGTGCTTTGAACTCGAGTTCAAAGCACTGTTAATGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCATTAACAGTGCTTTGAACTCGAGTTCAAAGCACTGTTAATGGGC-3'

Other clones with same target sequence:

(none)