Construct: shRNA TRCN0000011707

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145242.1-962s1c1
DNA Barcode:
CCTGCCAGTTGTTTGCTTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LOC115131 (115131)
Gene Description:
similar to POSSIBLE GUSTATORY RECEPTOR CLONE PTE01
Transcript:
RefSeq NM_145242.1 (NON-CURRENT)
Match location:
Position 962 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000011707)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGCCAGTTGTTTGCTTATT
Hairpin Sequence:
5'-CCGG-CCTGCCAGTTGTTTGCTTATT-CTCGAG-AATAAGCAAACAACTGGCAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGCCAGTTGTTTGCTTATTCTCGAGAATAAGCAAACAACTGGCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGCCAGTTGTTTGCTTATTCTCGAGAATAAGCAAACAACTGGCAGG-3'

Other clones with same target sequence:

(none)