Construct: shRNA TRCN0000011811

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021308.1-3222s1c1
DNA Barcode:
CCATCCTTGTAGAATTAGATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Piwil2 (57746)
Gene Description:
piwi-like RNA-mediated gene silencing 2
Transcript:
RefSeq NM_021308.1 (CURRENT)
Match location:
Position 3222 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000011811)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATCCTTGTAGAATTAGATT
Hairpin Sequence:
5'-CCGG-CCATCCTTGTAGAATTAGATT-CTCGAG-AATCTAATTCTACAAGGATGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATCCTTGTAGAATTAGATTCTCGAGAATCTAATTCTACAAGGATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATCCTTGTAGAATTAGATTCTCGAGAATCTAATTCTACAAGGATGG-3'

Other clones with same target sequence:

(none)