Construct: shRNA TRCN0000011856

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009412.1-512s1c1
DNA Barcode:
CCACAGCCAACGCTACCAGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tpd52 (21985)
Gene Description:
tumor protein D52
Transcript:
RefSeq NM_009412.1 (NON-CURRENT)
Match location:
Position 512 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000011856)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACAGCCAACGCTACCAGTA
Hairpin Sequence:
5'-CCGG-CCACAGCCAACGCTACCAGTA-CTCGAG-TACTGGTAGCGTTGGCTGTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACAGCCAACGCTACCAGTACTCGAGTACTGGTAGCGTTGGCTGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACAGCCAACGCTACCAGTACTCGAGTACTGGTAGCGTTGGCTGTGG-3'

Other clones with same target sequence:

  1. TRCN0000284923