Construct: shRNA TRCN0000011873

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008363.1-2484s1c1
DNA Barcode:
CAGCCAAAGAACTTGATAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Irak1 (16179)
Gene Description:
interleukin-1 receptor-associated kinase 1
Transcript:
RefSeq NM_008363.1 (NON-CURRENT)
Match location:
Position 2484 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000011873)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGCCAAAGAACTTGATAGAA
Hairpin Sequence:
5'-CCGG-CAGCCAAAGAACTTGATAGAA-CTCGAG-TTCTATCAAGTTCTTTGGCTG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGCCAAAGAACTTGATAGAACTCGAGTTCTATCAAGTTCTTTGGCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGCCAAAGAACTTGATAGAACTCGAGTTCTATCAAGTTCTTTGGCTG-3'

Other clones with same target sequence:

(none)