Construct: shRNA TRCN0000011903

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_181796.1-378s1c1
DNA Barcode:
CGGCAAATATGGCACCATGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gstp2 (14869)
Gene Description:
glutathione S-transferase, pi 2
Transcript:
RefSeq NM_181796.1 (NON-CURRENT)
Match location:
Position 378 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000011903)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGCAAATATGGCACCATGAT
Hairpin Sequence:
5'-CCGG-CGGCAAATATGGCACCATGAT-CTCGAG-ATCATGGTGCCATATTTGCCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGCAAATATGGCACCATGATCTCGAGATCATGGTGCCATATTTGCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGCAAATATGGCACCATGATCTCGAGATCATGGTGCCATATTTGCCG-3'

Other clones with same target sequence:

(none)