Construct: shRNA TRCN0000011924

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133753.1-1317s1c1
DNA Barcode:
CCCAAGTATGTCAGCAGCAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Errfi1 (74155)
Gene Description:
ERBB receptor feedback inhibitor 1
Transcript:
RefSeq NM_133753.1 (CURRENT)
Match location:
Position 1317 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000011924)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAAGTATGTCAGCAGCAAA
Hairpin Sequence:
5'-CCGG-CCCAAGTATGTCAGCAGCAAA-CTCGAG-TTTGCTGCTGACATACTTGGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAAGTATGTCAGCAGCAAACTCGAGTTTGCTGCTGACATACTTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAAGTATGTCAGCAGCAAACTCGAGTTTGCTGCTGACATACTTGGG-3'

Other clones with same target sequence:

(none)