Construct: shRNA TRCN0000011930

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_053110.2-1157s1c1
DNA Barcode:
CCTCTTTAATGCCTACTGGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gpnmb (93695)
Gene Description:
glycoprotein (transmembrane) nmb
Transcript:
RefSeq NM_053110.2 (NON-CURRENT)
Match location:
Position 1157 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000011930)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCTTTAATGCCTACTGGTT
Hairpin Sequence:
5'-CCGG-CCTCTTTAATGCCTACTGGTT-CTCGAG-AACCAGTAGGCATTAAAGAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCTTTAATGCCTACTGGTTCTCGAGAACCAGTAGGCATTAAAGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCTTTAATGCCTACTGGTTCTCGAGAACCAGTAGGCATTAAAGAGG-3'

Other clones with same target sequence:

  1. TRCN0000294524