Construct: shRNA TRCN0000011985

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019477.2-1300s1c1
DNA Barcode:
GCAAGGTTCAAGAGATGAATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Acsl4 (50790)
Gene Description:
acyl-CoA synthetase long-chain family member 4
Transcript:
RefSeq NM_019477.2 (NON-CURRENT)
Match location:
Position 1300 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000011985)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAAGGTTCAAGAGATGAATT
Hairpin Sequence:
5'-CCGG-GCAAGGTTCAAGAGATGAATT-CTCGAG-AATTCATCTCTTGAACCTTGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAAGGTTCAAGAGATGAATTCTCGAGAATTCATCTCTTGAACCTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAAGGTTCAAGAGATGAATTCTCGAGAATTCATCTCTTGAACCTTGC-3'

Other clones with same target sequence:

  1. TRCN0000297179